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. Author manuscript; available in PMC: 2021 Apr 24.
Published in final edited form as: Cell Rep. 2021 Mar 9;34(10):108828. doi: 10.1016/j.celrep.2021.108828

Figure 6. The M domain does not regulate UNC-13 protein level and localization.

Figure 6.

(A) Representative confocal z stack images for mApple-tagged UNC-13MR, UNC-13R, MUNC2C, and M-MUNC2C (all driven by the unc-129 promoter). Scale bar, 5 μm.

(B) Quantification of the fluorescence intensity from the lines in (A).

(C) Representative confocal z stack images for UNC-13MR and UNC-13R (tagged with mApple), and their colocalization with UNC-10/RIM (tagged with GFP). Scale bar, 5 μm.

(D) Quantification of the Pearson correlation coefficient between UNC-13MR/UNC-13R and UNC-10.

(E) Representative confocal z stack images for UNC-13MR::mApple, MRΔC1ΔC2B::mApple, UNC-13R::mApple, and RΔC1ΔC2B::mApple.

(F) Quantification of the fluorescence intensity from the lines in (E).

Data are means ± SEM. *p < 0.05, n.s., non-significant, one-way ANOVA. The number of worms analyzed for each genotype is indicated in the bar graphs.