KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and virus strains | ||
| Escherichia coli OP50 | Caenorhabditis Genetics Center (CGC) | OP50 |
| Chemicals, peptides, and recombinant proteins | ||
| CaCl2 | Fluka Analytical | Cat#21114 |
| MgCl2 | Fluka Analytical | Cat#63020 |
| NaCl | Sigma-Aldrich | Cat#S9888 |
| NaHCO3 | Sigma-Aldrich | Cat#S6014 |
| Sucrose | Sigma | Cat#S9378 |
| KCl | Sigma | Cat#P5405 |
| NaH2PO4 | Sigma-Aldrich | Cat#S0751 |
| Glucose | Sigma | Cat#G8270 |
| CsCl | Sigma | Cat#C3309 |
| CsF | Aldrich | Cat#289345 |
| EGTA | Sigma | Cat#E3889 |
| HEPES | Sigma | Cat#H4034 |
| Na2ATP | Sigma-Aldrich | Cat#2383 |
| CsOH | Aldrich | Cat#C8518 |
| Agarose | Sigma | Lot #SLBR6299V |
| All trans-Retinal | Sigma-Aldrich | CAS #116–31-4 |
| Experimental models: organisms/strains | ||
| C. elegans: N2 | Caenorhabditis Genetics Center | N2 |
| C. elegans: unc-13(s69) | Caenorhabditis Genetics Center | BC168 |
| C. elegans: hztSi1 [Psnb-1::UNC-13MR]; unc-13(s69) | This paper | ZTH456 |
| C. elegans: juSi67 [Prgef-1::UNC-13R]; unc-13(s69) | Zhou et al., 2013 | CZ15872 |
| C. elegans: hztEx11 [Psnb-1::UNC-13MR]; unc-13(s69) | This paper | ZTH11 |
| C. elegans: hztEx20 [Psnb-1::UNC-13R]; unc-13(s69) | This paper | ZTH99 |
| C. elegans: hztEx53 [Psnb-1::UNC-13MRΔC1]; unc-13(s69) | This paper | ZTH498 |
| C. elegans: hztEx68 [Psnb-1::UNC-13RΔC1]; unc-13(s69) | This paper | ZTH521 |
| C. elegans: hztEx55 [Psnb-1::MRΔC2B]; unc-13(s69) | This paper | ZTH517 |
| C. elegans: hztEx69 [Psnb-1::UNC-13RΔC2B]; unc-13(s69) | This paper | ZTH507 |
| C. elegans: hztEx70 [Psnb-1::MRΔC1ΔC2B]; unc-13(s69) | This paper | ZTH495 |
| C. elegans: hztEx109 [Psnb-1::UNC-13RΔC1ΔC2B]; unc-13(s69) | This paper | ZTH696 |
| C. elegans: hztEx54 [Psnb-1::UNC-13MRH348K]; unc-13(s69) | This paper | ZTH421 |
| C. elegans: hztEx56 [Psnb-1::UNC-13MRD3,4N]; unc-13(s69) | This paper | ZTH447 |
| C. elegans: hztEx72 [Psnb-1::UNC-13MRHK, D3,4N]; unc-13(s69) | This paper | ZTH451 |
| C. elegans: hztEx71 [Psnb-1::UNC-13RHK, D3,4N]; unc-13(s69) | This paper | ZTH448 |
| C. elegans: hztSi7 [Prab-3::UNC-13MUNC2C]; unc-13(s69) | This paper | ZTH858 |
| C. elegans: hztSi5 [Prab-3::UNC-13M-MUNC2C]; unc-13(s69) | This paper | ZTH752 |
| C. elegans: hztEx107 [Psnb-1::UNC-13MUNC2C]; unc-13(s69) | This paper | ZTH442 |
| C. elegans: hztEx108 [Psnb-1::UNC-13M-MUNC2C]; unc-13(s69) | This paper | ZTH577 |
| C. elegans: hztEx111 [Psnb-1:: NbMunc13−2-UNC-13R]; unc-13(s69) | This paper | ZTH738 |
| C. elegans: hztEx112 [Psnb-1::NbMunc13−2-UNC-13(MUNC2C)]; unc-13(s69) | This paper | ZTH740 |
| C. elegans: hztEx79 [Punc-129::UNC-13MR::mApple]; NuIs165 [Punc-129::UNC-10::GFP] | This paper | ZTH686 |
| C. elegans: hztEx80 [Punc-129::UNC-13R::mApple]; NuIs165 [Punc-129::UNC-10::GFP] | This paper | ZTH711 |
| C. elegans: hztEx82 [Punc-129::UNC-13MUNC2C::mApple]; NuIs165 [Punc-129::UNC-10::GFP] | This paper | ZTH693 |
| C. elegans: hztEx81 [Punc-129::UNC-13(M-MUNC2C)::mApple]; NuIs165 [Punc-129::UNC-10::GFP] | This paper | ZTH692 |
| C. elegans: hztEx113 [Psnb-1::NbMunc13−2(D145−187)-UNC-13R]; unc-13(s69) | This paper | ZTH924 |
| C. elegans: hztEx95 [Punc-129::UNC-13MRΔC1ΔC2B::mApple]; NuIs165 [Punc-129::UNC-10::GFP] | This paper | ZTH921 |
| C. elegans: hztEx66 [Punc-129::UNC-13RΔC1ΔC2B::mApple]; NuIs165 [Punc-129::UNC-10::GFP] | This paper | ZTH702 |
| Oligonucleotides | ||
| Unc-13 ΔC1 Forward: CAACTCAAAGAGCAGCCGAGAAGTCG | IDT | N/A |
| Unc-13 ΔC1 Reverse: TGCTCTTTGAGTTGTTGCACTGATCGGGTAGA | IDT | N/A |
| Unc-13 ΔC2B Forward: CAAATGGAAGGGAGAAGAGAAACTTGCACC | IDT | N/A |
| Unc-13 ΔC2B Reverse: CTCCCTTCCATTTGCTACTTCCTTCCAAAATAG | IDT | N/A |
| Unc-13 C2B D3,4N Forward: aagttcgagtttggaacgagaataatgatttgaaatc | IDT | N/A |
| Unc-13 C2B D3,4N Reverse: gatttcaaatcattattctcgttccaaactcgaactt | IDT | N/A |
| Unc-13 C1 HK Forward: caactcctaagaacttcgcaactacaacattc | IDT | N/A |
| Unc-13 C1 HK Reverse: gttgcgaagttcttaggagttgttgcac | IDT | N/A |
| Recombinant DNA | ||
| Psnb-1::UNC-13MR | This paper | Phzt11 |
| Psnb-1::UNC-13R | This paper | Phzt90 |
| Psnb-1::UNC-13MRΔC1 | This paper | Phzt387 |
| Psnb-1::UNC-13RΔC1 | This paper | Phzt523 |
| Psnb-1::UNC-13MRΔC2B | This paper | Phzt229 |
| Psnb-1::UNC-13RΔC2B | This paper | Phzt103 |
| Psnb-1::UNC-13MRΔC1ΔC2B | This paper | Phzt409 |
| Psnb-1::UNC-13RΔC1ΔC2B | This paper | Phzt501 |
| Psnb-1::UNC-13MRH348K | This paper | Phzt92 |
| Psnb-1::UNC-13MRD3,4N | This paper | Phzt356 |
| Psnb-1::UNC-13MRHK, D3,4N | This paper | Phzt367 |
| Psnb-1::UNC-13RHK, D3,4N | This paper | Phzt369 |
| Prab-3::UNC-13MUNC2C | This paper | Phzt746 |
| Prab-3::UNC-13M-MUNC2C | This paper | Phzt934 |
| Psnb-1::UNC-13MUNC2C | This paper | Phzt352 |
| Psnb-1::UNC-13M-MUNC2C | This paper | Phzt652 |
| Psnb-1:: NbMunc13−2-UNC-13R | This paper | Phzt943 |
| Psnb-1::NbMunc13−2-UNC-13(MUNC2C) | This paper | Phzt935 |
| Punc-129::UNC-13MR::mApple | This paper | Phzt1152 |
| Punc-129::UNC-13R::mApple | This paper | Phzt217 |
| Punc-129::UNC-13MUNC2C::mApple | This paper | Phzt925 |
| Punc-129::UNC-13(M-MUNC2C)::mApple | This paper | Phzt926 |
| Psnb-1::NbMunc13−2(D145−187)-UNC-13R | This paper | Phzt1071 |
| Punc-129::UNC-13MRΔC1ΔC2B::mApple | This paper | Phzt1173 |
| Punc-129::UNC-13RΔC1ΔC2B::mApple | This paper | Phzt1018 |
| Software and algorithms | ||
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| PatchMaster | HEKA Elektronik | V2×73.2 |
| Igor Pro | Wavemetrics | Version 7 |
| SigmaPlot | Systat Software Inc. | Version 13.0 |
| Other | ||
| Microscope Cover Glass | Fisher Scientific | Lot#060214–9 |
| Glass Capillaries | World Precision Instruments, Inc. | Lot#2009330 |