Table 2.
Relevant engineering strategies performed in Saccharomyces cerevisiae for terpenoid production (adapted from [7]).
| Compound | Titer | Strategy | References |
|---|---|---|---|
| Amorpha-4,11-diene | >40 g/L | Overexpression of ADS, upc2-1 Integration of genes copies using control of galactose-inducible promoters: 3 copies of tHMG1, and ERG10, 13, 12, 8, and IDI1 Deletion of gal80Δ Downregulation of ERG9 |
[60] |
| Artemisinic acid | 25 g/L | Overexpression of ADS, CYP71AV1, CPR1, CYB5, ALDH1 and ADH1 from A. annua, HEM1, and CTT1 under control of galactose-inducible promoters Deletion of gal80Δ Downregulation of ERG9 |
[27] |
| Farnesene | 130 g/L | Overexpression of ADA, xPK, PTA, NADH-HMGr, Farnesene synthase Deletion of adh1 Δ, ald4 Δ, ald6Δ, gpp1Δ, gal2Δ, bdh1Δ Overexpression of enzymes of the MVA pathway to Erg20 Downregulation of ERG9 |
[26] |
| Bisabolone | >900 mg/L | Overexpression of tHMG1, ERG20, upc2-1 and bisabolene synthase Downregulation of ERG9 |
[66] |
| Alpha-Santalene | 92 mg/L | Overexpression of tHMG1 Deletion of Ipp1Δ, dpp1Δ Downregulation of ERG9 |
[61] |
| Patchoulol | 42.1 mg/L | Overexpression of ERG20 and PatTps177 Downregulation of ERG9 |
[67] |
| (S)-Linalool | 0.26 mg/L | Overexpression of Erg20 and (S)-linalool synthase Diploid |
[68] |
| Geraniol | 1.69 g/L | 2μ plasmid of PTEF1-tVoGES-(GGGS)-ERG20WW fusion protein 2μ plasmid of PTEF1-tHMG1, PPGK1-IDI1, PTEF1- upc2.1 PHXT1-ERG20, oye2Δ |
[69] |
| Sabinene | 1.75 mg/L | 2μ plasmid of PTDH3-ERG20 (F96W-N127W)- sabinene synthase (Salvia pomifera) fusion protein Diploid ERG9/erg9, ERG20/erg20, PGal1-HMG2 (K6R), PTDH3-HMG2 (K6R) × 2 |
[70] |