Fig. 4.
A. TEER measurement setup uses a 4-point probe method in which the voltage-sensing electrodes are connected to a Millicell-ERS Volt-Ohm Meter. B. (a) The immunostaining images indicated a high-level tight junction protein (claudin-5 and ZO-1) expression showing continuous networks of ZO-1 (green) and claudin-5 (red) outlining the contours of BMECs. (b) The plot of TEER through the culture days obtained from the setup shown above. (c) The simulated plot of the average and maximum shear stresses with respect to step chamber height, (d) simulated average shear stress shows a linear correlation with h−2. Reproduced from [21] with the permission of Wiley. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)