Fig. 2.

Generation of Xa13 promoter mutants using CRISPR/Cas12a editing vectors. a Schematic of the T-DNA region of the editing vectors 24259 and 24277. b Comparison of transformation frequency between two vectors. c Deletion sizes derived by LbCas12a in 67 T0 lines from two constructs; bp, base pairs. d Different deletions occurred at the expected target sites, that is, the 5′-terminal second, third, and fourth nucleotides of the UPTPthXo1 box in the Xa13 promoter in the 5 T0 events that were selected as candidates for further resistance evaluation