Figure 1.

Plasma oxidative stress, adiponectin and cortisol determination. (A) The antioxidant capacity was evaluated using the BAP Test (Biological Antioxidant Potential), and (B) the levels of reactive oxygen metabolites using the d-ROM test (diacron-Reactive Oxygen Metabolite) by a free radical analyzer system (FREE Carpe Diem, DIACRON INTERNATIONAL s.r.l). (C) A representative immunoblot of adiponectin is shown together with the corresponding Coomassie-stained PVDF membrane. (D) Cortisol levels measured using enzyme-linked immunosorbent assay kit. All the measurements (n = 10 Control and 10 basketball) were performed in triplicate and are reported in the histograms as mean ± SD. The statistical analysis was carried out by two-tailed t-test using Graphpad Prism 8 (** p < 0.01; **** p < 0.0001).