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. 2021 Apr 3;11(4):1011. doi: 10.3390/ani11041011

Figure 2.

Figure 2

Post treatment progressive motility of liquid extended ram semen samples (n = 8) co-incubated (15 °C) with different concentrations of Fe3O4 and Ag/Fe nanoparticles (NPs) for 0, 30, 45, or 60 min. Control group (C): Extended ram semen samples-no treatment; iron oxide groups (Fe): Extended ram semen supplemented with different concentrations of Fe3O4 NPs (Fe ×2, Fe ×4, Fe ×8, Fe ×16, Fe ×32 correspond to 0.384 mg, 0.768 mg, 1.536 mg, 3.072 mg, and 6.144 mg of Fe3O4 NPs/mL semen, respectively); silver groups (Ag/Fe): Extended ram semen samples supplemented with different concentrations of Ag/Fe NPs (Ag/Fe ×2, Ag/Fe ×4, Ag/Fe ×8, Ag/Fe ×16, Ag/Fe ×32 corresponds to 0.256 mg, 0.512 mg, 1.024 mg, 2.048 mg and 4.096 mg of Ag/Fe NPs/mL semen, respectively). Values are expressed as LSmeans ± standard error of the mean (SEM). Differences at statistical level between groups are noticed by different superscripts (a, b, c, d). Significant differences between evaluation times within each experimental group are depicted by different symbols (*, #, +, ^).