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. 2021 Apr 3;11(4):1011. doi: 10.3390/ani11041011

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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CASA (computer assisted sperm analysis) motility and kinetics variables of liquid extended ram semen (n = 21) preserved at 15 °C for 6 and 24 h post treatment with Fe₃O₄ or Ag/Fe nanoparticles (NPs). Control group (C): Extended ram semen samples-no treatment; iron oxide group (Fe): Extended ram semen with Fe₃O₄ NPs (3.072 mg Fe₃O₄ per mL of semen); silver group (Ag): Extended ram semen with Ag/Fe NPs (2.048 mg Ag/Fe per mL of semen). Values are expressed as LSmeans ± standard error of the mean (SEM). Differences at statistical level between groups are noticed by different superscripts (a, b). Significant differences between evaluation times within each experimental group are depicted by different symbols (#, *).