Figure 1.
La Crosse virus (LACV) replicates efficiently and causes high mortality rates in suckling mice. In this study, two-day-old suckling mice (n = 16–23/group) were challenged intracranially with 103 plaque forming units (PFU) and had (a) survival and (b) weight change measured daily for 14 days. Only the phosphate buffered saline (PBS) uninfected control mice survived beyond four days postinfection (DPI). (c) Virus was detected in brain tissues harvested three DPI, and lineage III strains presented with the lowest titers among the lineages studied. Each data point plotted represents the mean values, and error bars indicate standard deviation. Statistical significance among groups and PBS controls was analyzed by log-rank (Mantel-Cox) test in (a) two-way ANOVA with Tukey’s multiple comparison test in (b,c) Statistical significance is denoted by * (p < 0.05).