Table 3.
Effect on toxin production by BCAAs.
| Culture Medium | pH | OD600 | TcdB (ng/mL) |
|---|---|---|---|
| TYfor | 7.0 | 1.0 | 1067 (904–1200) (n = 4) |
| TYfor, BCAA (10 mM) a | 7.0 | 0.76 | 622 (359–811) (n = 3) |
| TYfor, BCAA (25 mM) a | 7.0 | 0.86 | 766 (568–959) (n = 6) |
| TYfor, BCAA (40 mM) a | 7.0 | 0.91 | 630 (572–851) (n = 4) |
| TYfor, BCAA (100 mM) a | 7.0 | 0.67 | 181 (142–221) (n = 2) |
All cultures were incubated unagitated for 5 days at 37 °C and OD600 were measured on day 5. a The concentration of BCAA specifies the concentration of isoleucine, leucine, and valine added to the culture medium. for = tryptone (Formedium).