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Effect of buffering TYfor with PBS or PBS/bicarbonate on toxin production.
| Culture Medium | Solvent | pH | OD600 | TcdB (ng/mL) |
|---|---|---|---|---|
| TYfor | H2O | 7.0 | 0.82 | 943 (834–1051) (n = 2) |
| TYfor | PBS | 7.0 | 0.90 | 1112 (1001–1223) (n = 2) |
| TYfor | PBS/bicarbonate | 7.0 | 0.79 | 305 (235–375) (n = 2) |
Bicarbonate and PBS were added at 5 g/L and 100 mM, respectively. All cultures were incubated unagitated for 5 days at 37 °C, and OD600 was measured on day 5. for = tryptone (Formedium).
