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. 2021 Mar 27;13(4):240. doi: 10.3390/toxins13040240

Table 5.

Effect of pH on toxin production.

Culture Medium pH OD600 TcdB (ng/mL)
TYfor 6.5 0.70 594 (498–729) (n = 3)
TYfor 7.0 0.78 1160 (946–1214) (n = 3)
TYfor 7.5 0.85 1350 (1324–1376) (n = 3)
TYfor 8.0 0.89 849 (775–922) (n = 3)

TYfor culture media were buffered with 100 mM PBS. All cultures were incubated unagitated for 5 days at 37 °C, and OD600 was measured on day 5. for = tryptone (Formedium).