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Effect on toxin production by adding stationary-phase culture supernatant.
| Culture Medium | pH | OD600 | TcdB (ng/mL) |
|---|---|---|---|
| TYfor | 7.5 | 0.54 | 1024 (1020–1095) (n = 3) |
| TYfor, culture supernatant | 7.5 | 0.64 | 946 (850–1097) (n = 3) |
TYfor culture media were buffered with 100 mM PBS (pH 7.5). Stationary-phase culture supernatant was added at (33.3%, v/v). All cultures were incubated unagitated for 5 days at 37 °C with the OD600 measured on day 5. for = tryptone (Formedium).
