Table 9.
Comparison of incubation time.
| Culture Medium | Incubation (Days) | pH | OD600 | TcdB (ng/mL) |
|---|---|---|---|---|
| TYfor, 1 mM ZnCl2, 10 mM glucose | 1 | 7.5 | 1.77 | 1172 (1106–1398) (n = 3) |
| TYfor, 1 mM ZnCl2, 10 mM glucose | 3 | 7.5 | 1.15 | 3155 (2985–3318) (n = 3) |
| TYfor, 1 mM ZnCl2, 10 mM glucose | 5 | 7.5 | 0.86 | 3136 (3114–3340) (n = 3) |
TYfor culture media were buffered with 100 mM PBS (pH 7.5), and ZnCl2 was filtrated into the culture flasks after media was autoclaved. All cultures were incubated unagitated for 5 days at 37 °C, while samples were taken on days 1, 3, and 5 and measured for OD600 and TcdB concentration. for = tryptone (Formedium).