Table 10.
Effect of various metal salts on toxin production
| Culture Medium | pH | OD600 | TcdB (ng/mL) |
|---|---|---|---|
| TYfor | 7.5 | 1.19 | 1501 (1485–1508) (n = 3) |
| TYfor, 5 mM ZnCl2, 10 mM glucose | 7.5 | 0.84 | 4385 (3576–4683) (n = 3) |
| TYfor, 5 mM FeSO4, 10 mM glucose | 7.5 | 1.23 | 346 (327–580) (n = 3) |
| TYfor, 5 mM CuSO4, 10 mM glucose | 7.5 | 0.23 | 10 (9–11) (n = 3) |
| TYfor, 5 mM MnCl2, 10 mM glucose | 7.5 | 1.03 | 1272 (1190–1453) (n = 3) |
| TYfor, 5 mM MgCl2, 10 mM glucose | 7.5 | 0.86 | 1722 (1649–2059) (n = 3) |
TYfor culture media were buffered with 100 mM PBS (pH 7.5) and incubated unagitated for 3 days at 37 °C with OD600 measured on day 3. All metal salts were filtrated into the culture flasks after media was autoclaved. for = tryptone (Formedium).