Table 11.
Effect of medium optimization in large-scale dialysis tube cultures.
| Culture Medium | Volume (mL) | pH | OD600 | TcdB (ng/mL) |
|---|---|---|---|---|
| TYfor (flask) | 50 | 7.5 | 0.82 | 1046 (1039–1054) a (n = 3) |
| TYopt (flask) | 50 | 7.5 | 0.85 | 4429 (3779–4433) b (n = 3) |
| TYfor (dialysis tube) | 425 | 7.5 | 5.5 | 8868 (7728–10,401) (n = 5) |
| TYopt (dialysis tube) | 400 | 7.5 | 5.3 | 29,500 (28,983–34,029) (n = 4) |
TYfor culture media were buffered with 100 mM PBS (pH 7.5) and incubated unagitated for 3 days at 37 °C with OD600 measured on day 3. for = tryptone (Formedium). opt = Optimized TYfor culture medium buffered with PBS (pH 7.5), 5 mM ZnCl2 and 10 mM glucose. a Result from Table 1. b Result from Table 8.