Table 12.
Summary of all conditions that increased toxin production.
| TcdB Expression | Condition | Fold Increase |
|---|---|---|
| Culture medium | Tryptone/yeast extract (TYfor) | 1 |
| Buffering | 100 mM PBS, pH 7.5 | 1.3 |
| Metal salt | 5 mM ZnCl2 | 2.7 |
| Metal salt, sugar | 5 mM ZnCl2, 10 mM glucose (TYopt) | 4.1 |
| Scale-up (dialysis tube) | 5 L culture medium a | 28.2 |
for = tryptone (Formedium). opt = Optimized TYfor culture medium buffered with PBS (pH 7.5), 5 mM ZnCl2 and 10 mM glucose. a Dialysis tube culture immersed in 5 L Pyrex® Erlenmeyer flask.