Figure 9.

Modulatory effects of MJe on mRNA levels of Parkinson-related genes modulated by 6-OHDA. SH-SY5Y cells were pretreated with MJe for 1 h and then exposed to 6-OHDA 50 µM for additional 24 h. Messenger RNA levels were quantified with real-time PCR, and their relative quantities were calculated through the 2^–ΔΔCT method. Results are expressed as fold change relative to untreated cells. Data are expressed as mean ± SEM of three separate experiments in triplicate (N = 9). * p < 0.05, *** p < 0.001 vs. control; ° p < 0.05, °° p < 0.01 and °°° p < 0.001 vs. 6-OHDA 50 µM.