Table 8.

Molecular markers selected for genotyping of the barley F₂ population.

Marker	Class	Oligo Forward	Oligo Reverse	Tm	D Allele	R Allele	Chr
Bmac 0310	SSR	CTACCTCTGAGATATCATGCC	ATCTAGTGTGTGTTGCTTCCT	55 °C	176 pb	138 pb	4
Bmag 0211	SSR	ATTCATCGATCTTGTATTAGTCC	ACATCATGTCGATCAAAGC	55 °C	187 pb	198 pb	1
HVM40	SSR	CGATTCCCCTTTTCCCAC	ATTCTCCGCCGTCCACTC	55 °C	175 pb	146 pb	4
Knox-dup	PCR	CCATGTTGCTGTATTTTGCG	ACTGCACTGCAACTGGTCAG	60 °C	325 pb	-	4

Chr: chromosome, D allele: allele presents in the OWB-D parental line, R allele: allele presents in the OWB-R parental line.