Figure 5.
RVA NSP1 inhibits IRF-1. HEK-293 cells were transfected with a plasmid containing a firefly luciferase gene under the control of the PRDIII-I element from the IFN-β promoter, a plasmid directing constitutive expression of renilla luciferase as an internal control, a plasmid encoding IRF-1 where indicated, and either the empty vector pcDNA3.1, or pcDNA3.1 encoding the NSP1 protein from the indicated RVA strains, or CSFV Npro; 48 h after transfection, cells were lysed and levels of firefly and renilla luciferase were measured. The normalised activity of the reporter gene in the sample transfected with the empty vector and IRF-1 was set to 100%. Differences between induced samples containing NSP1 and the induced control sample containing the empty vector were assessed using the Student’s t-test (**** p < 0.0001).