Table 4.
Robustness of the UHPLC method developed.
| Column Temperatures (°C) | Flow Rates (mL min−1) | Injection Volumes (µL) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 52 | 55 | 58 | 0.57 | 0.60 | 0.63 | 2.8 | 3.0 | 3.2 | ||
| Retention time (min) | Quercetin 7,3,4′-O-triglucoside | 1.026 a | 0.945 b | 0.882 c | 1.026 a | 0.945 b | 0.912 c | 0.955 a | 0.945 a | 0.929 a |
| Quercetin 7,4′-O-diglucoside | 1.501 a | 1.477 a | 1.497 a | 1.590 a | 1.477 b | 1.540 b | 1.530 a | 1.477 a | 1.559 a | |
| Quercetin 3,4′-O-diglucoside | 1.797 a | 1.687 b | 1.679 b | 1.748 a | 1.687 b | 1.627 c | 1.694 a | 1.687 a | 1.674 a | |
| Isorhamnetin 3,4′-O-diglucoside | 1.936 a | 1.835 b | 1.804 b | 1.887 a | 1.835 b | 1.775 c | 1.839 a | 1.835 a | 1.823 a | |
| Quercetin 3′-O-glucoside | 2.231 a | 2.119 b | 2.002 c | 2.161 a | 2.119 b | 2.056 c | 2.126 a | 2.119 a | 2.111 a | |
| Quercetin 4′-O-glucoside | 2.591 a | 2.478 b | 2.357 c | 2.512 a | 2.478 a | 2.417 b | 2.484 a | 2.478 a,b | 2.471 b | |
| Isorhamnetin 4′-O-glucoside | 2.797 a | 2.693 a | 2.574 b | 2.721 a | 2.692 b | 2.633 c | 2.697 a | 2.693 a,b | 2.683 b | |
| Peak area | Quercetin 7,3,4′-O-triglucoside | 1584 a | 1749 a | 1992 a | 1815 a | 1749 a | 1846 a | 1493 a | 1749 a | 2270 b |
| Quercetin 7,4′-O-diglucoside | 4073 a | 4459 a | 5070 a | 5733 a | 4459 a | 5004 a | 3246 b | 4459 a,b | 5251 b | |
| Quercetin 3,4′-O-diglucoside | 104,048 a | 112,705 a | 123,675 a | 123,884 a | 112,705 a | 122,463 a | 88,187 a | 112,705 a | 140,119 b | |
| Isorhamnetin 3,4′-O-diglucoside | 5073 a | 5245 a | 5931 a | 5892 a | 5245 a | 5800 a | 4233 a | 5245 a | 6905 b | |
| Quercetin 3′-O-glucoside | 5045 a | 4899 a | 5396 a | 5330 a | 4899 a | 5448 a | 4015 a | 4899 a,b | 5811 b | |
| Quercetin 4′-O-glucoside | 151,175 a | 162,031 a | 178,379 a | 175,285 a | 159,754 a | 173,719 a | 130,086 a | 165,384 a | 198,383 b | |
| Isorhamnetin 4′-O-glucoside | 18,933 a | 20,539 a | 22,383 a | 22,187 a | 20,539 a | 21,882 a | 16,308 a | 20,539 a | 25,220 b | |
| Peak resolution | Quercetin 7,3,4′-O-triglucoside | - | - | - | - | - | - | - | - | - |
| Quercetin 7,4′-O-diglucoside | 6.22 a | 7.69 a | 6.94 a | 6.95 a | 7.69 a | 7.68 a | 7.62 a | 7.69 a | 8.15 a | |
| Quercetin 3,4′-O-diglucoside | 2.89 a | 2.15 a,b | 1.6 b | 1.62 a | 2.15 a | 0.810 b | 1.80 a | 2.15 b | 1.20 a | |
| Isorhamnetin 3,4′-O-diglucoside | 1.92 a | 1.93 a | 1.59 a | 2.09 a | 1.93 a | 1.95 a | 2.10 a | 1.93 a | 1.94 a | |
| Quercetin 3′-O-glucoside | 4.54 a | 4.56 a | 2.9 b | 4.60 a | 4.72 a | 4.56 a | 4.48 a | 4.56 a | 4.44 a | |
| Quercetin 4′-O-glucoside | 3.30 a | 3.68 a | 3.47 a | 3.70 a | 3.67 a | 3.68 a | 3.43 a | 3.68 a | 3.82 a | |
| Isorhamnetin 4′-O-glucoside | 1.87 a | 2.23 b | 2.11 a,b | 2.14 a | 2.23 a | 2.15 a | 2.10 a | 2.23 a | 2.16 a | |
Same letter in the same row means that there were no significant differences as per the t-test (p < 0.05).