Table 3.

Adjusted comparison of circulating acylcarnitine and fatty acid levels among groups.

A. ANCOVA 1 (Sex, Age, HOMA-IR, eGFR)
	noPH-HF vs. Controls	PH-HF vs. Controls	PH-HF vs. noPH-HF
Acylcarnitines
Free carnitine	<0.01	<0.001	NS
C2-AC	<0.01	<0.001	<0.05
C16-AC	<0.05	<0.01	NS
C18:1-AC	<0.01	<0.001	0.07
C18:2-AC	<0.01	<0.001	NS
Saturated fatty acids
C20:0	NS	NS	<0.05
C22:0	<0.01	<0.05	NS
C24:0	<0.001	<0.001	NS
Monounsaturated fatty acids
C16:1Tn7	NS	NS	NS
C18:1n7	NS	<0.05	<0.01
C18:1n9	NS	<0.05	<0.01
Polyunsaturated fatty acids
C18:2n6	<0.05	NS	NS
C20:4n6	NS	NS	<0.05
C20:5n3	<0.001	<0.01	NS
C22:5n3	<0.05	NS	<0.05
C22:4n6	NS	<0.05	<0.05
C22:6n3	<0.001	<0.05	NS
B. ANCOVA 2 (Sex, Age, HOMA-IR, eGFR, NT-proBNP)
	noPH-HF vs. Controls	PH-HF vs. Controls	PH-HF vs. noPH-HF
Acylcarnitines
Free carnitine	<0.05	<0.01	NS
C2-AC	NS	<0.01	0.06
C16-AC	NS	<0.05	NS
C18:1-AC	<0.01	<0.001	0.07
C18:2-AC	<0.05	<0.001	NS
Saturated fatty acids
C20:0	NS	NS	<0.05
C22:0	NS	NS	NS
C24:0	<0.001	<0.001	NS
Monounsaturated fatty acids
C16:1Tn7	NS	<0.05	NS
C18:1n7	NS	<0.05	<0.01
C18:1n9	NS	<0.01	<0.01
Polyunsaturated fatty acids
C18:2n6	NS	NS	NS
C20:4n6	NS	NS	<0.05
C20:5n3	<0.01	NS	NS
C22:5n3	NS	NS	NS
C22:4n6	NS	NS	NS
C22:6n3	<0.05	NS	<0.05

Pre-specified variables included the ANCOVA 1 model were age, sex, eGFR, HOMA-IR; ANCOVA 2 adjusted for age, sex, eGFR, HOMA-IR and NT-proBNP. NT-proBNP was log-transformed. ANCOVA denotes analysis of covariance; eGFR, estimated glomerular filtration rate; HOMA-IR, homeostatic model assessment of insulin resistance; NS, non significant. p-values are derived from the ANCOVA model and refer to the comparison of plasma metabolites among groups.