Table 1.

Flow program used for silver nanoparticle (AgNP) analysis by AF4. Detection flow: 0.5 mL·min⁻¹. * For assays to study the polydispersity of the aggregated AgNPs. For more explanations see text.

	Time, min	Direct Flow, mL·min−1	Crossflow, mL·min−1	Focus Flow, mL·min−1
Focus	7	0.2	1.0 (0.2) *	1.3
Transitional	0.5	0.2 to 1.5	1.0 (0.2) *	1.3 to 0
Fractionation	35	1.5 to 0.5	1.0 (0.2) * to 0	0
	10	0.5	0	0