Table 1.

Primers used for the introduction of stop codons into glycoprotein genes.

Primer	Sequence (5′-3′)
UL55stop_for	ggaatccaggatctggtgcctggtagtctgcgttaacttgtgaatcgtccgtctgggttaagcggtttcctcatcttctacaggatgacgacgataagtaggg
UL55stop_rev	gagtagcagaagttccacgagtagaagatgaggaaaccgcttaacccagacggacgattcacaagttaacgcagactaccacaaccaattaaccaattctgattag
UL55stop_short_for	ggaatccaggatctggtgcc
UL73stop_for	taagcatcgtggcggtggtgtgatggagtggaacacactatgattaggtcttttggtttaatcggtagtggcaagttccaaaggatgacgacgataagtaggg
UL73stop_rev	tgctagcagtcgacgtattgttggaacttgccactaccgattaaaccaaaagacctaatcatagtgtgttccactccatcacaaccaattaaccaattctgattag
UL73stop_short_for	taagcatcgtggcggtggtg
UL73stop_for M3	caacgtgatgagaccacatgctcacaatgatttttacaattgacattgtacatcgcattagtatgagctttcactgtccagaggatgacgacgataagtaggg
UL73stop_rev M3	tattccaccaggctgcaaagctggacagtgaaagctcatactaatgcgatgtacaatgtcaattgtaaaaatcattgtgagcaaccaattaaccaattctgattag
UL73stop_for_short M3	caacgtgatgagaccacatg
UL75 stop_for	cgctatgcggcccggcctccccttctacctcaccgtcttctaggtctacctccttagttgactaccttcgcaacgatatggaggatgacgacgataagtaggg
UL75 stop_rev	cttcggatgcggcgtctgcgccatatcgttgcgaaggtagtcaactaaggaggtagacctagaagacggtgaggtagaaggcaaccaattaaccaattctgattag
UL75 stop_short_for	cgctatgcggcccggcctcc
UL100stop_for	cgtggactttgaaaggctcaacatgtcggcctacaacgtatgacacctgcacacgccttaacttttcttagactcggtgcaaggatgacgacgataagtaggg
UL100stop_rev	acacggcgtagcacaccaactgcaccgagtctaagaaaagttaaggcgtgtgcaggtgtcatacgttgtaggccgacatgtcaaccaattaaccaattctgattag
UL100stop_for_short	cgtggactttgaaaggctca
UL115stop_for	ctctcatcgtgccgcagacttgatgtgccgccgcccggattgaggcttctctttctcataaggaccggtggtactgctgtgaggatgacgacgataagtaggg
UL115stop_rev	tgggcagcagaaggcaacaccacagcagtaccaccggtccttatgagaaagagaagcctcaatccgggcggcggcacatcacaaccaattaaccaattctgattag
UL115stop_short_for	ctctcatcgtgccgcagact
Kanamycin universal reverse	caaccaattaaccaattctga

Primer sites encoding stop codons are underlined.