Figure 8.

Pretreatment with anti-HMGB1 antibodies protects auditory hair cells in noise-exposed cochlea. (A) Representative images of a surface preparation of the basal and middle turns of the cochlea of the pretreated and untreated control groups on day 28 after noise exposure. Immunofluorescence staining shows the nuclei (blue, DAPI) and filamentous actin (green, phalloidin). (B) The survival rates of the outer hair cells in the basal and middle turns of mouse cochleae from each group. The results are expressed as the mean ± SEM (n = 4 [refers to 4 cochleae from 4 different animals] for each bar). Scale bars = 50 μm. * p < 0.05; DAPI = 4,6-diamidino-2-phenylindole; SEM = standard error of the mean.