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. 2021 Mar 26;10(4):519. doi: 10.3390/antiox10040519

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The fertility of aged oocytes is improved by nicotinamide (NAM) supplementation in Caenorhabditis elegans: (A) The percentage of embryonic lethality among the total number of progenies produced by young, aged, and NAM-treated aged (NAM+aged) N2 hermaphrodites. Error bars represent standard deviation (SD). *, p < 0.05 (one-way ANOVA with Tukey’s post hoc test); (B) The number of unfertilized oocytes (upper graph) and small embryos (bottom graph) among the total number of progenies of young, aged, and NAM+aged N2 hermaphrodites. Representative images of unfertilized oocytes (indicated by yellow arrows) and a small embryo (indicated by a white arrow). Error bars represent SD. *, p < 0.05 (one-way ANOVA with Tukey’s post hoc test).