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. 2021 Mar 26;10(4):519. doi: 10.3390/antiox10040519

Figure 4.

Figure 4

Mitochondrial activity and oxidative stress responses in offspring produced by aged oocytes are improved by maternal nicotinamide (NAM) supplementation in Caenorhabditis elegans: (A) Comparison of mitochondrial activity in the F1 generation produced by young, aged, and NAM+aged mothers using MitoTracker staining. The graph shows the relative levels of mitochondrial activity by ImageJ analysis. Error bars represent standard deviation (SD). *, p < 0.05 (one-way ANOVA with Tukey’s post hoc test); (B) Comparison of mitochondrial reactive oxygen species (ROS) levels in the F1 generation produced by young, aged, and NAM+aged mothers using CellROX Green staining. The graph shows the relative levels of mitochondrial ROS by imageJ analysis. Error bars represent SD. *, p < 0.05 (one-way ANOVA with Tukey’s post hoc test); (C,D) The F1 generation of HSP-6::GFP and SOD-3::GFP transgenic animals produced by young, aged, and NAM+aged mothers, and respective fusion proteins were observed under fluorescence microscopy and quantified using ImageJ (each condition, n = 20). Error bars represent SD. *, p < 0.05 (one-way ANOVA with Tukey’s post hoc test).