Figure 2.

Mild inhibition of BRV on delayed-rectifier K⁺ current (I_K(DR)) in GH₃ cells. In these experiments, the cells were bathed in Ca²⁺-free Tyrode’s solution containing 1 µM tetrodotoxin and 0.5 mM CdCl₂, where the recording electrode was filled with a K⁺-containing solution comprising: K-aspartate 130 mM, KCl 20 mM, MgCl₂ 1 mM, Na₂ATP 3 mM, Na₂GTP 0.1 mM, EGTA 0.1 mM, and HEPES 5 mM adjusted to pH 7.2 with KOH. (A) Representative I_K(DR) traces obtained in the absence (upper) and presence of 10 µM BRV. The inset in the upper part of the figure indicates the voltage profile used; (B) Comparison of the mean current-voltage (IV) of I_K(DR) without (○) or with the addition (■) of 10 µM BRV (mean ± SEM; n = 8). The current amplitude was measured at the end of each voltage command pulse. It should be noted that BRV (30 µM) mildly inhibited the I_K(DR) amplitude at +50 mV.