Figure 5.

Process of differentiation of H-iris iPS cells to nervous system cells. Cells were pre-differentiated from (a) H-iris iPS cells to (b) neural stem/progenitor cells by adhesive culture. (c) Cells sorted with p75NTR were differentiated into neurons. By changing the medium condition, the nerve cells were differentiated into Recoverin-positive cells. (d) In contrast, after suspension culture, neurites were elongated by adhesive culture and differentiated into retinal ganglion cells. Bars: 100 μm.