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. 2021 Mar 28;10(4):743. doi: 10.3390/cells10040743

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunostaining of cells differentiated into nerve cells. Fluorescent immunostaining was performed with the following markers of neural stem/progenitor cells: (a) Musashi, (b) Nestin, (c) Merge of Musashi and Nestin. Immunostaining was then performed with the following markers of nerve cells: (d) Neurofilament-H, (e) Tubulin-βIII, (f) Merge of Neurofilament-H and Tubulin-βIII, (g) Neurofilament-M, (h) MAP2, (i) Merge of Neurofilament-M and MAP2. Bars in panels (a–f): 50 μm, bars in panels (g–i): 100 μm.