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. 2021 Mar 28;10(4):743. doi: 10.3390/cells10040743

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cells sorted with p75NTR can concentrate Recoverin-positive cells. (a) Before p75NTR sorting, cells with large cytoplasms (arrowheads) were mixed. (b) Fixed cells were analyzed for p75NTR and Nestin by FCM. Most of the cells sorted in the strong positive region of p75NTR were double-positive for (c) p75NTR and (d) Nestin. (e,f) The morphology of cells sorted by their strong positivity for (e) p75NTR or (f) their weak positivity or negativity for p75NTR. (g) Relative semi-quantitative analysis of Recoverin gene expression in cells selected by p75NTR, and pre/post-differentiated cells. (h) Fluorescent immunostaining of Recoverin in differentiated cells. Bar in panel (a): 50 μm, bars in panels (c,d): 20 μm, bars in panels (e,f,h): 100 μm.