Skip to main content
. 2021 Apr 1;10(4):679. doi: 10.3390/plants10040679

Figure 2.

Figure 2

Figure 2

TwMYC2a/b could bind to the E-box (CACATG) and T/G-box (CACGTT) in yeast cells. (A) Schematic diagram of the different E-box motifs present in the TwTPS27a and TwTPS27b promoters. These motifs are E1 (CAGATG), E2 (CACATG), E3 (CAAATG), E4 (CACGTT, called T/G-box), and E5 (CAATTG), respectively. ATG, the start codon. (B) Schematic diagrams of the bait and prey vectors used for yeast one-hybrid (Y1H) assays. The triple repeated sequences of E-box (E2, CACATG) and T/G-box (E4, CACGTT) were synthesized and ligated into pAbAi, while the full-length coding sequences of TwMYC2a and TwMYC2b were separately fused with GAL4 AD in pGADT7-Rec2. (C) Y1H assays showing that TwMYC2a/b could bind to the E-box (E2, CACATG) and T/G-box (E4, CACGTT) present in the TwTPS27a/b promoters, based on the ability of the transformed Y1HGold yeast cells to grow on SD/−Leu/AbA200 and SD/−Leu/AbA300 medium in the gradient dilution (1/10, 1/100, 1/1000). The transformants grown on SD/−Leu/−AbA plate were used as positive controls for transformants growth. Positive transformants were confirmed by spotting yeast cells onto agar medium of SD/−Leu with 200 and 300 ng/mL AbA. These assays were repeated three times with similar results.