Figure 1.

Frequency of regulatory T (T_reg) cells did not change in patients with rheumatoid arthritis (RA). Blood samples were collected from healthy donor (HC, n = 13) and rheumatoid arthritis patients (RA, n = 13) and analyzed by flow cytometry. (A) Flow cytometry gating scheme of T_reg subpopulations in human peripheral blood mononuclear cells (PBMC). FMO (fluorescence minus one control); HC (healthy control). Percentage of (B) CD4⁺ T cells among CD3⁺ T lymphocytes in PBMC, (C) CD25⁺, Foxp3⁺, or CD25⁺Foxp3⁺ T_reg cells among CD4+ T cells, and (D) CD25⁺CD127^−/low, or CD25⁺CD127^−/low Foxp3⁺ T_reg cells among CD4⁺ T cells. Data from individual subjects were presented with the median values. Statistical differences were calculated by Mann–Whitney test.