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. 2021 Apr 1;10(4):785. doi: 10.3390/cells10040785

Figure 2.

Figure 2

(a) Type I interferon (IFN) pathway activation in THP1-Dual™ cells after cGAMP and LPS challenges (n = 4 independent experiments). THP1-Dual™ cells were pre-treated for 24 h with cGAMP (0.5–1.25–2.5–5–10–20 μg/mL), and afterward stimulated for 1 h with LPS (0.5 μg/mL). Luminescence values were normalized on the untreated condition. (b) Type I IFN production in bone marrow-derived dendritic cells (BMDCs) after cGAMP and LPS challenges (n = 3 independent experiments). BMDCs were pre-treated for 24 h with cGAMP (0.025–0.5–1 μg/mL) and afterward stimulated for 16 h with LPS (1 μg/mL). Secreted type I IFN was evaluated by B16-Blue IFN-α/β™ reporter cells assay. Results are reported as mean ± SEM. Statistically significant p-values are shown (Two-way ANOVA: **** p < 0.0001).