Figure 2.

Temperature tracings comparing the experience of flight and terrestrial control samples throughout the duration of the missions. HOBO temperature sensors were incorporated into selected GAPs and retrieved during hardware de-integration at the end of the mission. (A) Samples in the Micro-6 payload that were activated early in the SpX CRS-1 mission, (B) Samples in the Micro-8 payload that were activated and then terminated late in the SpX CRS-4 mission, and (C) Samples in the Micro-14/FPA payload that were activated and ‘terminated’ with fresh medium late in the SpX CRS-16 mission for return of viable cells. Temperature sensors were not associated with FEP bag bioreactors in SpX CRS-17. Terrestrial control operations were performed near synchronously (2 h delay from flight) and for the purpose of this illustration the time offset was removed from the tracings to allow overlap of transitions for better comparison. Flight temperatures are traced in black, terrestrial control temperatures are traced in green, and key operations are indicated in text and highlighted with red vertical lines.