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. 2021 Mar 27;11(4):283. doi: 10.3390/life11040283

Figure 4.

Figure 4

Passive seeding of biofilm communities on siliconized rubber membrane coupons. Siliconized rubber membrane coupons were included in the medium (chamber A) of selected FPAs in Micro-14/FPA and allowed to freely seed during the 24 h growth period of the experiment. Samples were fixed in microgravity and following sample return and hardware de-integration, the membranes were recovered and stained with Crystal Violet as an indicator of cells, cellular debris, and extracellular matrix. Stain that is retained on the membrane through a series of three water washing steps (wash 1, 2 and 3) and a final acetic acid wash (aa wash) is suggestive of a tighter integration of the cells with the substrate material. The stain removed by each wash was quantified by optical density at 595 nm. Black line = flight; green line = terrestrial control.