Table 1.

Asymmetrical flow field-flow fractionation (AF4) separation conditions applied in the release experiments. The injection and detector flow were set to 0.2 mL/min and 1 mL/min, respectively.

Mode	Duration, min	Cross/Focus Flow Start, mL/min	Cross/Focus Flow End, mL/min
Elution	1	1	1
Focus	1	2	2
Focus and inject	5	2	2
Focus	3	2	2
Elution	10	2	2
Elution	35	0	0
Elution and inject	5	0	0