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. 2021 Mar 27;10(4):741. doi: 10.3390/cells10040741

Figure 1.

Figure 1

RhoGEF17 is essential for cell-cell contacts and adherens junctions (AJ) protein regulation in EC. (A,B,D) Human umbilical vein endothelial cells (HUVEC) were transduced with adenoviruses encoding EGFP alone (EGFP) or in addition to a shRNA against RhoGEF17 (sh17-1). (A) After 48 h, RhoGEF17 was detected in cell lysates. Shown are representative immunoblots of RhoGEF17 and α-tubulin and the quantitative analysis. Values are normalized and given as means + SEM with the single data points, n = 7, * p < 0.05 analyzed by paired t-testing. (B). Depicted are bright field/EGFP overlay images of transduced HUVEC. Scale bar = 100 µm. (C) Rat fat pad endothelial cells (RFPEC) were transduced for 48 h and then used to generate spheroids. Bright field and fluorescence images are shown. Scale bar = 200 µm. (D) VE-cadherin, p120-catenin, and α-tubulin were detected by immunoblot in lysates of transduced HUVEC. Shown are representative immunoblots and the quantified data normalized by α-tubulin and relative to EGFP as means + SEM with the single data points, n = 4 − 7, * p < 0.05 analyzed by paired t-testing.