Fig. 4.

Alteration of abundance of 157 metagenome-assembled genomes (MAGs) detected in HR biofilms at mesophilic (M) and thermophilic (T) process temperature in response to the increase of organic loading rate (OLR) from 500 to 1500 g ryegrass silage as deduced from metagenome data. PCA plots are shown for principle component analyses of scaled and centered rlog transformed metagenome read counts mapped on MAGs (a). The hierarchical clustering of rlog transformed abundance values for 78 (contamination below 10%) selected MAGs detected in HR biofilms is visualized in (b). Green, mesophilic HR biofilms; blue, thermophilic HR biofilms. For further details on sample denomination, refer to Figs. 2 and 3. Alteration in abundance values of 78 MAGs selected in response of the increase of OLR from 500 to 1500 g ryegrass silage at mesophilic (c) and thermophilic (d) process temperature. Colors of the MAGs indicate taxonomic affiliation (green, Firmicutes; blue, Bacteroidota; violet, Archaea; pink, Cloacimonadota; light green, Fibrobacterota; dark red, Spirochaetota; red, unknown affiliation). * indicate MAGs with completeness above 50% and contamination rate less than 10% as listed in Table 1 and in Additional file 4