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Comparison of duration of activin A treatment (72 and 168 h) on the differentiation of the iPSC line Windy (feeder-free) into the endoderm. (a) Human iPSC-derived endoderm differentiation protocols, namely 72 h activin A and 168 h activin A. (b) Relative gene expression levels of OCT4, SOX17, FOXA1, and GATA4. Human iPSCs = 1. Results are presented as means ± S.D. (n = 3). Levels of statistical significance: *** p < 0.001.
