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. 2021 Apr 6;10(4):812. doi: 10.3390/cells10040812

Figure 3.

Figure 3

A comparison of differentiated endoderm on the pretreatment or absence of 1% DMSO using 72 h activin A and ACP protocols. (a) Human iPSC-derived endoderm differentiation method using 72 h activin A and ACP protocols in the presence or absence of 1% DMSO. (b) Relative gene expression levels of undifferentiated cell markers and endodermal markers in the human iPSC line Windy-derived endoderm using 72 h activin A and ACP protocols with or without pretreatment with 1% DMSO. Results are presented as means ± S.D. (n = 3). Human iPSCs = 1. Levels of statistical significance: * p < 0.05, *** p < 0.001.