Figure 6.

A comparison of the human iPSC line Windy cultured with or without feeder cell-derived endoderm during hepatocyte differentiation. (a) Relative gene expression of ALB, BSEP, and CYP3A4. The mRNAs of cryopreserved human primary hepatocytes were collected at 0 and 48 h of culture. (b) Immunofluorescence images of albumin (red), MRP2 (green), and AFP (red). Nuclei were stained with DAPI (blue). (c) CYP3A4/5 activity in the presence or absence of 10 μM ketoconazole, a CYP3A4 inhibitor. Scale bars = 100 μm. All data are presented as mean ± S.D. (n = 3). Levels of statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.