Figure 4.

Enrichment analysis of direct and indirect mRNA interactions and proteins dysregulated in penile cancer (PeCa) cells show EGFR as a core molecule. Sensitivity assays to EGFR inhibitors are depicted for cells 2 and 3. Upstream regulator analysis (IPA, Ingenuity Pathway Analysis) indicates direct (solid lines) and indirect (dashed lines) interaction of mRNA (A) and proteins, which is exemplified in PeCa-derived cell 2. (B) EGFR is a core molecule in both mRNA and protein analyses. (C) Chemosensitivity assays were performed in cell 2 and cell 3 for the identification of IC50 and dose-response curves. Cells were treated with cisplatin and EGFR inhibitors (cetuximab, gefitinib, and erlotinib). Cell 2 and cell 3 were sensitive to cisplatin. Only cell 2 (EGFR overexpression) was sensitive to EGFR inhibitors.