Viability of AML cell lines treated with Nut-3a and/or WIP1i. (A) Percentage of viable MOLM-13, MV-4-11, OCI-AML3, NOMO-1, HEL and KASUMI-1 AML cells treated with increasing concentrations of single agent Nut-3a (from 0.5 to 5 μM) for 24, 48 and 72 h. (B) IC50 values of AML cell lines at 72 h of treatment with Nut-3a or WIP1i (NR = not reached). (C) Percentage of viable cells treated with increasing concentrations of single agent WIP1i (from 5 to 20 μM) for 24, 48 and 72 h. Inhibition of cell viability induced in TP53-wt (D) and TP53-mut (E) AML cell lines by the combination of increasing concentrations of Nut-3a (from 0.5 to 5 μM) and WIP1i (from 5 to 20 µM) at 24, 48 and 72 h. Average value and standard deviation of 3 independent experiments are shown.