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. 2021 Apr 6;10(4):816. doi: 10.3390/cells10040816

Figure 11.

Figure 11

Effects of 5FU, gemcitabine and doxorubicin on colony formation of MIA-PaCa-2 + pLXSN, MIA-PaCa-2 + WT-GSK-3β, and MIA-PaCa-2 + KD-GSK-3β cells. The effects on colony formation in response to 5-FU (Panel (A)), gemcitabine (Gem), (Panel (B)) and doxorubicin (Dox) (Panel (C)) treatment on MIA-PaCa-2 + pLXSN (solid green bars), MIA-PaCa-2 + WT-GSK-3β (solid red bars), and MIA-PaCa-2 + KD-GSK-3β (solid blue bars). The MIA-PaCa-2 + WT-GSK-3β, MIA-PaCa-2 + KD-GSK-3β, and MIA-PaCa-2 + pLXSN cells in each panel were all examined at the same time period. In each condition, the cells were plated in 3 wells of a 6 well plate. The colony formation abilities were determined three times for each cell type and each treatment condition and similar results were observed. *** p < 0.0001, ** p < 0.005, and * p < 0.05.