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Effects of combining a constant concentration of 250 nM berberine on the tideglusib IC50. Tideglusib by itself (red squares) or tideglusib and a constant dose of 250 nM berberine (blue triangles on: (A) MCF-7 + pLXSN; (B) MCF-7 + WT-GSK-3β; and (C) MCF-7 + KD-GSK-3β cells). The experiments in (A–C) were all performed on the same day. They were repeated 3 times and similar results were observed. *** p < 0.0001.
