Skip to main content
. 2021 Apr 6;11(4):264. doi: 10.3390/membranes11040264

Figure 3.

Figure 3

Cell surface distribution of PlyA2-EGFP (green) and lipid raft markers (red). Fixed HeLa cells were doubly immunolabeled with PlyA2-EGFP and antibodies against raft-associated glycosylphosphatidylinositol-anchored protein CD59 and ganglioside GM3. Alternatively, cells were labelled with non-raft transferrin followed by fixation and PlyA2-EGFP labelling. Arrows, partial co-localization between PlyA2-EGFP and CD59. Based on dot-by-dot comparisons, about 45% of CD59-positive dots co-localized with PlyA2-EGFP. In contrast, only 5% of the non-raft transferrin receptor was visualized with plasma-membrane-bound Alexa-labelled transferrin. Ganglioside GM3 did not show significant co-localization with SM/Chol domains labeled by PlyA2-EGFP. Adapted from [38].