Table 1.

List of primers and PCR conditions used in this work. Endonuclease restriction sites added for cloning purposes in some primers are underlined.

Primer Name	Sequence (5′-3′)	Cycling Conditions (°C/min) a	Purposes
wbaP_KKBO-1_AvaI	TAACGCTCGAGCGGTGTCCCAGTAAAAGG	D(94/1) A(52/1) E(72/1)	Cloning in pGEM-T-Easy®; Sequencing
wbaP_KKBO-1_EcoRI	AAGCAGAATTCACGCCAAATATCACCACCAT		Cloning in pGEM-T-Easy®; Sequencing
Seq_wbaP_fwd	GTGATGGCGGTGTTCCTG		Sequencing; Screening
Seq_wbaP_rev	GGTAGCCACGACAAATC		Sequencing; Screening
pACYC184_ExtSeq_Ava	GCTAACGGATTCACCACT		Sequencing; Screening
pACYC184_ExtSeq_EcoRIrev	CCTTTATTCACATTC		Sequencing; Screening

^a D stands for denaturation, A stands for annealing, and E stands for extension. All reactions include an initial denaturation step of 5 min at 94 °C and a final extension step of 5 min at 72 °C.