Figure 2.

Protective effects of EEBC against H₂O₂-damaged HT22 neuronal cells. (a) Cells were incubated with various concentrations of EEBC for 24 h and cell viability evaluated using a Cell Counting Kit (CCK) assay; (b) Cells were co-treated with EEBC (0, 12.5, 25, or 50 μg/mL) and H₂O₂ (250 μM) for 6 h. CCK assay was performed to assess changes in cell viability. Caveolin was used as a positive control. Data are expressed as % of the control. Values represent mean ± SEM. ^### p < 0.001 vs. untreated cells; * p < 0.05 and ** p < 0.01 vs. H₂O₂-treated cells; (c) Morphological changes in cells were observed under inverted microscopy (Eclipse TS100, Nikon, Japan). EEBC: ethanol extract of B. coccinea branches. CCK: Cell Counting Kit. PC: positive control.