Skip to main content
. 2021 Apr 6;10(4):702. doi: 10.3390/plants10040702

Figure 3.

Figure 3

Free radical scavenging activity of EEBC. (a) Various concentrations of EEBC (0, 3.125, 6.25, 12.5, 25, 50, or 100 μg/mL) were reacted with equal volumes of ABTS solution for 5 min in the dark at room temperature. Absorbance of the reactants was read at 734 nm using a spectrophotometer; (b) Various concentrations of EEBC (0, 3.125, 6.25, 12.5, 25, 50, or 100 μg/mL) were reacted with equal volumes of DPPH solution for 30 min in the dark at room temperature. Absorbance of the reactants was read at 517 nm using a spectrophotometer. Ascorbic acid (AA, 5 μg/mL) was used as a positive control. Each value is presented as the mean ± SEM. ** p < 0.01 and *** p < 0.001 vs. control. EEBC: ethanol extract of Bauhinia coccinea branches. ABTS: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid). DPPH: 2,2-diphenyl-1-picrylhydrazyl.